Effect of Mutant EDA-A1 Gene on Huvecs

Effect of Mutant EDA-A1 Gene on Huvecs Effect of EDA-A1 gene mutant on proliferation and cell cycle distribution of cultured human umbilical vein endothelial cell Running title: The effect of mutant EDA-A1 gene on HUVECs. Ke Lei, MM; Lunchang Wang, MD; Bing Ma, MM; Ping Shi, MD; Longjiang Li, MD; Tuanjie Che, MD; Xiangyi He, MD Highlights: EDA-A1 gene mutant significantly decreased proliferation of human umbilical vein endothelial cells (HUVECs). HUVECs of mutant group were blocked at G0/G1 and S phase. HUVECs of wild group accumulated in S phase and decreased in G2/M phase. Abstract Background: To investigate the effect of ectodysplasin A gene (EDA-A1) on proliferation and cell cycle of human umbilical vein endothelial cells (HUVECs) and explore the possible mechanism underlying this process. Methods: Recombinant eukaryotic expression vectors pcDNA3.1(-)-EDA-A1-M/W (mutant, M; wild, W) containing the coding sequence of EDA-A1-M/W were transfected into HUVECs. EDA-A1-M/W genes were amplified by reverse transcription polymerase chain reaction (RT-PCR), and the proteins were detected by western blot. Then MTT assay for cell proliferation of HUVECs in each group was performed and cell cycle was detected using flow cytometry. Results: The EDA-A1 gene and protein were detected respectively by RT-PCR and western blot in HUVECs transfected with pcDNA3.1(-)-EDA-A1-M/W, but not in HUVECs transfected with empty plasmid pcDNA3.1(-) (control group) and cells without transfection. Compared with control group, EDA-A1 gene mutant significantly decreased proliferation of HUVECs and the inhibition rate was 45.70% (PEDA-A1 gene did not cause such growth inhibition (P>0.05). A significant increase of the G0/G1 and S fraction was seen in the HUVECs of mutant group, compared with wild group with an accumulation in S phase and a concomitant decrease in G2/M phase population (P Conclusion: Compared with the wide-type, the mutant EDA-A1 gene could inhibit the proliferation and cell cycle of the HUVEC. Key words: EDA-A1 gene; Mutant; Human umbilical vein endothelial cell; Cell cycle; Proliferation Introduction Hypohidrotic ectodermal dysplasia (HED), also called anhidrotic ectodermal dysplasia (AED) or Christ-Siemens-Touraine Syndrome, is a kind of X-linked recessive genetic disease (XLHED) (1). HED is a rare congenital genetic disorder with a birth incidence of 1/100,000-1/10,000 (2, 3). It is characterized by the diminution or absence of eccrine sweat glands, oligodontia and peg shaped teeth and sparse hair (1, 4). Previous study indicates that XLHED is caused by the ectodysplasin A gene (EDA-A1) mutant (5). EDA-A1, a major causative gene of HED, locates in Xq12-13.1 and encodes a novel tumor necrosis factor (TNF) ligand family protein ectodysplasin A (EDA-A1) and this protein is associated with the nuclear factor-ÃŽ ºB (NF-ÃŽ ºB) signaling mechanisms (5-9). Bayes M et al. (10) indicates that the full-length of EDA-A1 is 5296bp (http://www.ncbi.nlm.nih.gov/, AH007059, Gene ID 4007891), the open reading frame (ORF) of EDA-A1 is 1176bp, and it encoding the protein with 391 amino acids (EDA-A1, GeneID1896). Studies showed the combination of EDA-A1 and ectodysplasin receptor (EDAR) could promote programmed cell death and active the signaling of NF-ÃŽ ºB (8, 11). Recently, the related research on HED are mostly for mutation analysis of EDA-A1, and more than 100 mutations in the EDA gene have been reported to cause XLHED up to now (12, 13). However, there have few reports relating to the function of mutant EDA-A1, and the exact pathological mechanism of mutant EDA-A1 on HED is still unclear. In the present study, EDA-A1 mutant (pcDNA3.1 (-)-EDA-A1-M) and wild type (pcDNA3.1(-)-EDA-A1-W) eukaryotic expression vector that we used were constructed in our previous study (14). Then the function of transfected EDA-A1 and its mutant for cell proliferation and cell cycle of HUVECs were analyzed. The aim of this study was to investigate the effect of EDA-A1 on proliferation and cell cycle of HUVECs and explore the possible mechanism underlying this process. Material and Method Cell culture HUVECs were kindly provided by professor Wang chunming (Lanzhou University, China). HUVECs were cultured in RPMI-1640 (Huamei Company, Shanghai, China) Medium. The medium were consisted of 10% fetal bovine serum (FBS) (Evergreen Company, Hangzhou) and 100U/ml penicillin/streptomycin. All these cells were maintained in humidified incubator of 5% CO2 at 37à ¢Ã¢â‚¬Å¾Ã†â€™ (0.25% trypsin digestion overnight). Inverted microscope was used for the cell morphology investigation. All the experiments were performed at least in triplicate and repeated at least twice. Plasmid extraction EDA-A1 mutant (pcDNA3.1(-)-EDA-A1-M) and wild type (pcDNA3.1 (-)-EDA-A1-W) eukaryotic expression vector that we used were constructed in our previous study (14). Totally 3ÃŽ ¼l mutant (M) and Wild-type (W) plasmid DNA was extracted respectively from transfected HUVECs, followed by the sterile deionized water diluted to 1ml. The values of à ¢Ã¢â€š ¬Ã¢â‚¬ ¹Ãƒ ¢Ã¢â€š ¬Ã¢â‚¬ ¹A260nm and A280nm were measured by UV spectrophotometer. Plasmid DNA concentration (ÃŽ ¼g / ÃŽ ¼l) = A260 Ãâ€" dilution factor Ãâ€" 50/1000. The plasmid DNA (positive recombinants and empty control) was precipitated by ethanol. Then the DNA pellet was resuspended in sterile deionized water. Cell transfection Cell transfection was carried out according to the instructions of QIAGEN-Effectene Transfection Reagent Kit (QIAGEN). Transfection was carried out when the cell density was up to 70% after 24 hour-cell passaging. Cells were transferred into a complete medium (CM) 2 hours before transfection. Totally 2.5 µg mutant (M) and Wild-type (W) plasmid DNA was slowly added to the 2 M CaCl2 solution (stand for 10 minutes). DNA-CaCl2 solution was slowly added dropwise to the 2 Ãâ€" HeBS (stand for 30 minutes) until the precipitation of tiny particles. The precipitate was uniformly dropwise added to the culture flasks. After a 12 hours growth under standard conditions, cells were washed 2 times with HeBS, followed by the cultured in CM. HUVECs transfected with empty vector were used as the control group. Semi-quantitative real-time PCR To identify the expression levels of EDA-A1 in HUVECs, semi-quantitative real-time PCR (SqRT-PCR) analysis was performed. Total RNA was extracted from cultured cells in each group (cultured for 48 hours) by using reverse transcription (RT) kit (Fermentas Company), followed by the EDA-A1 primers designation (Primer Premier 5.0 software) and synthesis (Shanghai Biological Engineering Company ). The primers used were as follows, EDA-A1 (408bp): 5’- CGC AGG ATC CAT GGG CTA CCC GGA GGT -3’ (forward) and 5’- ATT AAG CTT GCC AAG CGG GCA CCA GGG AGA C -3’ (reverse), ÃŽ ²-actin (230bp): 5’- ACG CAT TTG GTC GTA TTG GG-3’ (forward) and 5’- TGA TTT TGG AGG GAT CTC GC-3’ (reverse). The 50ÃŽ ¼l PCR reaction system were: cDNA template (2ÃŽ ¼l), 10 Ãâ€" PCR Buffer (5ÃŽ ¼l), dNTP (1ÃŽ ¼l), primer (up and downstream, 1ÃŽ ¼l), Taq DNA polymerase (1ÃŽ ¼l), ddH2O (39ÃŽ ¼l). Products were subjected to electrophoresis (1.5% agarose gel, 120V, 90mA). Western blot analysis For Western blot analysis, proteins were extracted from HUVECs in each group. Proteins were collected after cell lysis. Protein concentration was determined using the Bradford dye-binding method (15). The proteins were separated by SDS-PAGE and transferred to the 0.45ÃŽ ¼m pore size nitrocellulose (NC) membrane (RPN303E, Amersham Company). NC membranes were blocked with TBS buffer (5% milk and 0.5%-Tween) for 1 hour (37 °C). Then, the membrane was incubated overnight at 4à ¢Ã¢â‚¬Å¾Ã†â€™ with the rabbit antibodies EDA-A1 and ÃŽ ²-actin (1:200 dilution with TBST solution), followed by incubation at room temperature for 1h with an anti-rabbit secondary antibody (Sigma). Finally, the expression levels of the target proteins were visualized withchromogenic substrate. MTT assay for cell proliferation detection To determine the proliferation of HUVECs in each group, the 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay was performed. The 24 hours-transfected and untransfected cells were seeded into 96-well plate with inculation density of 5000 cells/well and incubated at 37à ¢Ã¢â‚¬Å¾Ã†â€™. After 12 hours, 100 ÃŽ ¼l serum-free DMEM was added in each well. After 72 hours, 20 ÃŽ ¼l MTT was added into each well to continue incubation at 37à ¢Ã¢â‚¬Å¾Ã†â€™(4 hours). Then, the medium was removed and the precipitation was dissolved in DMSO. The absorbance at 560 nm was measured by SpectraMax 190 microplate reader (Moteular Devices Company) for colorimetric analysis. Inhibition rate of cell growth was calculated (n=10) based on the experimentally measured absorbance value (OD value). Cell cycle analysis Flow cytometry was used to detect the cell cycle.After incubation for 48 h, the cells were collected and washed with cold PBS. The washed cells were fixed in 70% cold ethanol with incubation overnight at 4à ¢Ã¢â‚¬Å¾Ã†â€™. To stain the cells, prodium iodide (PI) solution was added. Flow cytometer (Coulter Epics XL, Beckman Coulter Company) was used to analyze the samples. Cell Quest software was used to analyze the cell percentage of G0 / G1 phase, S phase, and G2 / M phase. Statistical analysis All assays were performed in triplicate and datawere expressed as mean values  ±s.d. The SPSS 13.0 software employing ANOVA was used to analyze all data which expressed as mean ±SD. P values less than 0.05 was considered as significantly different. Results EDA-A1 expression pattern in HUVECs influenced by plasmid-mediated transfection To identify the expression level of ED1-A1 in HUVECs transfected with vector pcDNA3.1(-)-EDA-A1-M or pcDNA3.1(-)-EDA-A1-W, the RNA samples with an OD260/OD280 ration of 1.8-2.0 were chosen for RT-PCR. The HUVECs with pcDNA3.1(-)-EDA-A1-M or pcDNA3.1(-)-EDA-A1-W transfection showed a band nearly 400 bp compared with control using semi-quantitative PCR and primers specific to EDA-A1 (Figure 1). Additionally, ÃŽ ²-actin band between 200 bp and 300 bp have been seen in all the groups. Then, EDA-A1 protein expression in HUVECs were detected by western blot. Figure 1 shows that the EDA-A1 protein was expressed in the transfected cells with pcDNA3.1(-)-EDA-A1-M or pcDNA3.1(-)-EDA-A1-W vector, however, it could not be achieved in control group. In conclusion, the EDA-A1 was expressed in HUVECs after exogenous delivered of EDA-A1, but not in the un-treated control cells. Overexpression of EDA-A1 affects HUVECs proliferation To elucidate the effect of EDA-A1 on HUVECs proliferation, the MTT assays were performed. As shown in Figure 2, the HUVECs viability at 96 h transfection was decreased significantly in the mutant group by comparison with wild type and control. The proliferation of mutant group cells was suppressed by 45.7% compaired to control, while the wild type group was suppressed by 16.0% (Table 1, Figure 3). EDA-A1 overexpression regulates the cell cycle of HUVECs To determine the role of plasmid-mediated EDA-A1 transfection in cell cycle of HUVECs, the flow cytometry was used (Figure 4). We observed that 25.45  ± 1.89 % cells were arrested at G0/G1 phase of cell cycle in the mutant group compared with 20.37  ± 0.6% and 20.30  ± 0.68% cells in wild type and control groups, respectively (Table 2). During S phase, both mutant and wild type groups showed significantly higher cell percentages (14.80  ± 1.45% and 12.4 0  ± 1.75%) than that of control (8.55  ± 0.57%). However, both transfection groups had lower cell percentages than control in G2/M phase. The lowest cell percentage with 62.15  ± 1.94% was showed in the mutant group during S phase. We could conclude that the cell cycle distribution in G0/G1, S, and G2/M of HUVECs were regulated by EDA-A1 overexpression. Discussion HED characterized by impaired development of hair, eccrine sweat glands and teeth is caused by mutations in the EDA-A1 gene (3, 16). Recently, the related research on HED are focused on the mutation analysis of EDA-A1, however, the exact pathological mechanism of HED caused by mutant EDA-A1 is still unclear (17). In this study, we investigated the effect of HED related gene EDA-A1 on proliferation and cell cycle of HUVECs. The results showed that mutant EDA-A1 gene significantly decreased proliferation of HUVECs (P EDA-A1 protein, a type à ¢Ã¢â‚¬ ¦Ã‚ ¡ transmembrane protein, is one of the TNF ligand family members involved in ectodermal development (18). EDA-A1 contains a TNF-like domain (aa: 245–391), a collagen domain, and a furin protease recognition sequence (7, 8, 19-21). The TNF-like domain is necessary and sufficient for receptor molecule EDAR binding (22, 23). Furthermore, EDA-A1 has been shown to specifically bind to EDAR, which could promote programmed cell death and active the signaling of NF-ÃŽ ºB (8, 11). In our study, the reason why EDA-A1 mutant could inhibit the proliferation and block the cell cycle progression in G0/G1 phase and S phase of HUVECs might be the change of protein spatial configuration and biological activity that caused by the EDA-A1 gene mutation and the changed protein could not combined with EDAR and thus inhibit the signaling of NF-ÃŽ ºB. Maria et al. found that HED was related with the blocked signaling pathway of NF-ÃŽ ºB (9). Pascal et al. found th at point mutations in the TNF-like domain of EDA-A1 strongly decreased EDAR binding to EDA-A1 by altering the folding of EDA (21). Moreover, the substitution of Gln306 with Pro in our study was found to be located in the TNF-like domain of EDA-A1 and may influence the epithelial signaling pathway required for the normal ectodermal development through altering the topology of EDA, which is consistent with previous study. HUVECs are cells derived from the endothelium of veins from the umbilical cord, and they are often used as a laboratory model system for the study of the function and pathology of endothelial cells (24). Some studies showed that during vascular development and pathological angiogenesis, the maintenance of blood vessel homeostasis and its functional execution depend on the integrity of vascular endothelium, which is affected by proliferation, migration and apoptosis of endothelial cells (25, 26). Furthermore, Jie et al. showed that recovery of injured endothelial cells through regulated endothelial cell proliferation plays significant roles in thrombosis disease (27). In our study, mutant EDA-A1 decreased the proliferation of HUVECs, therefore, we suspected that pathological mechanism underlying HED caused by EDA-A1 may be the growth inhibit of endothelial cells which could lead to the defection of eccrine sweat glandsis. Despite of all results mentioned above, there were still some l imitations in the present study, whether the EDA-A1 mutant blocked the combination of EDA-A1 with EDAR required further experiment. In conclusion, our study revealed EDA-A1 gene mutant could inhibit the proliferation and cell cycle of HUVECs. We explored the mechanism of HED caused by mutant EDA-A1. The substitution of Gln306 with Pro may influence the epithelial signaling pathway required for the normal ectodermal development through altering the topology of EDA, which could impair the binding of EDA-A1 to EDAR and further inhibit the signaling of NF-ÃŽ ºB. Our finding broadens the spectrum of EDA-A1 mutations and may help to understand the molecular basis of XLHED and aid genetic counseling. Acknowledgements We wish to express our warm thanks to Fenghe(Shanghai) Information Technology Co., Ltd. Their ideas and help gave a valuable added dimension to our research. Conflict of interest The authors have declared that no competing interests exist. Authors’ contributions KL and LW participated in the design of this study, and they both performed the statistical analysis. BM and TC carried out the study, together with PS, collected important background information, and drafted the manuscript. LL and XH conceived of this study, and participated in the design and helped to draft the manuscript. All authors read and approved the final manuscript. Figure legends: Figure 1 Detection of mRNA expression of EDA-A1gene in ECV304 cells by RT-PCR: M: mutant group; W: wild group; C: control group. Figure 2 Expression of ECV304 cells transfected with EDA-A1 gene and mutant: M: mutant group; W: wild group; C: control group. Figure 3 OD560 value of ECV304 cells transfected with EDA-A1 gene after cultured for 96h: M: mutant group; W: wild group; C: control group; a: compared with the control group, P Figure 4 The effect of EDA-A1 gene mutant on cell cycle in ECV304 cells. Table 1 OD560 value of ECV cells transfected with EDA-A1 gene after cultured for 96h Note: a: compared with control group, P Table 2 Effect of EDA-A1 gene mutant on cell cycle in ECV304 cells Note: a: compared with control group, P

Functions of Doctrine in a Christian and African Perspective

Doctrine, in Ninian Smart’s dimensions of religion refers to the writings and textbook knowledge that people have regarding their religion. This is exactly what people believe about their respective religions passed on from the generation to generation. Doctrine is in fact, â€Å"the set of answers one has accepted to life’s profound questions. † It intrinsically comprises what the ethical standards of a certain religion would have and it â€Å"directly affects† the behavior of its followers. The â€Å"symbolic and mythical† are given order by doctrine, since people cannot rely on abstract truths alone.The main purpose of doctrine is to give â€Å"authoritative and sometimes systematic proofs that their religious reality and everyday reality is one and the same. † It may also be said that doctrines are somewhat answers laid out by the authoritative body in a certain religion, to answer the questions of life. They are â€Å"logical† hig hly systematized body of religious knowledge intrinsic in religion. It is evident that when two opposing doctrines clash, â€Å"believers commit the most bloody atrocities in the name of their belief† and in the process stay true to the doctrines they uphold.Certain aspects of the human life such as â€Å"death, suffering and change† are given light by religious doctrines, since it gives a foundation of belief to the religious follower. It may be said that people need something to hold on too, because these facts of life are much too grave for the ordinary human mind. In general, doctrines are â€Å"belief systems which provide answers to certain boundary/identity questions. † What cannot be explained is therefore given light by religious doctrines, and this help in a person’s acceptance of life and its realities.The functions then of doctrine are to â€Å"bring order and focus to myth and ritual†, â€Å"provide institutionalization of answers to the unexplainable†, â€Å"control boundaries of religious expression†, and â€Å"determine what is inclusive and exclusive in a given religion. † African religion has been greatly questioned by early European explorers visiting the continent. Some Africans were actually even converted to both Islam and Christianity through these explorers. In modern times, African religion still continues to be dominantly pagan.Dialogues regarding African religions have had certain difficulties since African â€Å"ethnic groups lack a term for religion in the Western sense as an entity or activity separate from everyday life. † Africans uphold religion as a complete way of life. African religion also may become misleading, since doctrines per tribe differ in degree and belief, where it is only called â€Å"doctrines† for formality purposes. As most world religions, the African religions hold that there is one creator of the universe who withdrew and remains remote f rom the concerns of the world.Believers do not directly talk to this god, nor do they offer sacrifices to him. Instead, secondary divinities are seen as ‘middlemen’ between them and their god. They are considered as â€Å"children of the god† or at least are held to be high ranking in their religious hierarchy. It should be noted that African â€Å"religions do not demand adherence to any single doctrine. † The intent of these religions is almost always pragmatic, where â€Å"religious rituals serve as strategies for reinforcing life, fertility and power. Doctrine then for them is a set of practice and rituals that they do in order for them to survive.What they believe in is that â€Å"the principal vision shared by African religions is that human beings must vigilantly maintain a harmonious relationship with divine powers in order to prosper. † The goal of these religions is to control these divine powers for the betterment of their lives, and â⠂¬Å"ritual is the way to do so†. A practical way of looking at religion and doctrine is what comprises African religions.Ritual then â€Å"ensures a community’s responsible relationship with ancestors who are guardians of the moral order, with spiritual forces within nature, and with the gods. † Of course, for Christianity, it is a totally different story. Doctrine for Christianity is a way of life, yet it is not purely for survival. Rituals are present yet not in the same sense as African religion where they offer animal sacrifices and hold mystical ceremonies. Christian doctrine therefore is an ethical way of life, a compilation of the facts about the religion, compiled by the Catholic Church.Christian doctrine therefore centers on the truth about God, Jesus and life. These are ways on how to live a Christian life which also teaches the way of Jesus. It may be seen as dogmatic, since the early doctrines were considered more of dogma than a religion that can hel p in a person’s salvation. These Catholic doctrines had been protested and continuously scrutinized by scientists and philosophers like Friedrich Nietzsche. The Catholic Church has been known in its early years to have imposed a way of life to the Christians, implementing soul and even physical persecution in the name of sin.The Catholic Church, though not changing the religious doctrines through time, adapts to the modern world of democracy and human rights. Persecution, like in Dante’s Inferno is not taught anymore, and was instructed to be uplifted because people follow not because they believe in the doctrine, but because of fear of persecution. Christian doctrine has now become a way of life for most Christians, deepening and rejuvenating their faith in their daily lives through it.

Reliable Tips for Disadvantaged Student Essay Samples You Can Use Starting Today

Reliable Tips for Disadvantaged Student Essay Samples You Can Use Starting Today The Lost Secret of Disadvantaged Student Essay Samples Equality even in punishment ought to be maintained such they aren't always sending to the director of disability services even as soon as the offence doesn't have anything to do with disability. There are various types of rules that have been set in various nations. There are several ways in which broken rules are dealt with because penalties are set on how best to deal with various rules. Aside from becoming laws some rule can always be changed if they're viewed as inappropriate and not providing the necessary results. Should you need further changes in your resume, we'll be happy to help you too through our absolutely free revision policies and straightforward money-back guarantee if your expectations aren't met. Let's consider advantages and pitfalls. We can't overlook the automated disadvantage accrued to disability but there's an opportunity to earn a positive impact if the proper strategy is engaged. Erikson felt in this stage that children would grow to be a self-sufficient human being or doubt anything that may do. Education is the largest game changer. The Art of Storytelling is the most recent installment in a set of completely free courses from the studio named Pixar in a Box. In years past a disabled child wouldn't be able to be taken to school and even worse, they'd be killed. The white student manages to receive a better score when compared with the black student. Working with these kinds of students requires communication. To find out more about what things to anticipate f rom the study of medicine, take a look at our Study Medicine in america section. To start with, be yourself. Advantage Disadvantage Essay Model Answer These days lots of people decide to call home or work in different nations, that has been made possible as a result of the ease of air travel and contemporary communications. A suitable studying to identify the requirements of the students needs to be done. Use active instead of passive language. New Ideas Into Disadvantaged Student Essay Samples Never Before Revealed For years, ethnic minority groups struggle to locate adequate work when compared with whites, though they are well educated and meet all the criteria necessary for the job. Social workers deliver various services like educating and advising patients and household members, different forms of counselling, making referrals to other services, organising support groups and several other services. These social workers try to maximize academic operation of children and improving the family general well-being. Public health social workers are liable for helping people who've been diagnosed with chronic diseases and disorders which is one of the most difficult things an individual could go through. The Appeal of Disadvantaged Student Essay Samples Within the subject of social work, there are numerous distinct places and people which they can pursue and focus on. People with disabilities are a part of the society and strategies to accommodate their existence has to be deliberated upon in order to help them catch up with the remainder of the society. Higher education is of wonderful price and benefit, but on the opposite hand, because all individuals differ based on their abilities, lifestyle and ideals that society does not have to target every citizen to get increased education, come what may. Living with disabilities demands a lot of resource and knowledge. Ideally, the should cover their education should bring about a more conscious selection of profession, in fact, first, man isn't always rational. There are various skills necessary for different specialisations. Apart from it gives new and a lot of job opportunities it assists the country to be globally known. In the facet of employment, it has opened plenty of job opportunities for those. Why Almost Everything You've Learned About Disadvantaged Student Essay Samples Is Wrong The rest well a lot of them don't pay anything. In a couple of cases where your story is very compelling, you always have the option to write about the exact same topic from other angles. Think freely, but you're not permitted to think of anything else besides the topic available. It's said that the 1 thing that nobody can ever take away from you is your education and that's the only thing I plan to not just gain for myself but for others also. If you own a topic or experience that's uniquely yours, you have the possibility of an interesting and memorable essay. Paid tuition is merely a possibility, the question is the best way to utilize it. Because of this, everybody should be in a position to pursue higher education. The initiative in creating change has ever been an integral facet of man. Possessing good essay examples provides the reader an in-depth and on-the-court idea about what a well structured and coherent essay appears like. Employing Native english speakers articles with the assistance of countable and also uncountable nouns may well come to be hard to comprehend. This writing company makes sure their papers are all the terrific quality and all the customers are pleased. Scroll down the page in order to look at extra essay samples which might help you in producing your very own literary essay. This is intended to make certain that if your paper incorporates references, they are finished based on the kind of preference. PaperCoach can assist you with all your papers, so take a look at the moment!

Thesis Tourism and Rizal Park - 7777 Words

CHAPTER 1 The Problem and Its Setting Introduction Rizal Park as everybody seen it today is the product of years of painstaking work by thousands of unknown citizens who gave of their time and their labors to create something of beauty where there was nothing but yawning wilderness in the very heart of the premier city. Its continued cleanliness and order is a tribute to the people who use it more than to those who tend to it. Here is a park that is used, loved and nurtured by the people who saw it shape up from nothing (http://rizalpark.nationalparks.ph/main.htm, October 9, 2013). Tourist attractions can be natural or man-made. The history and culture of a place are also very important attractions that enhance the natural†¦show more content†¦The researchers will identify the factors that influenced tourists to visit the destination. The result of this study will help promote the Rizal Park as the Face of the Philippine Ecotourism. This will give them knowledge on how to preserve and improve the destination to attract more local and foreign tourists. It will also give them accurate insights about the effects of the development of the park to tourists. This would be a big help in knowing the evaluation regarding the location, facilities, amenities, safety, security, and affordability, so that the people responsible on governing Rizal Park will be able to know the strengths, weaknesses, opportunities and threat of the particular site. Paradigm of the Study The paradigm illustrates the conceptual framework of the study, which illustrates how the specific objectives will be answered. This study will tend to find out about the Development of the new Rizal Park and its effects on the local and foreign tourists’ arrivals. Statement of the Problem This study aimed to determine the development of the new Rizal Park and its effects on the local and foreign tourist’s arrivals. Specifically, it sought answers to the following questions: 1. What is the profile of the respondents